منابع مشابه
Regulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase.
We have examined three mutants of Klebsiella aerogenes whose genetic lesions (glnB, glnD, and glnE) are in loci unlinked to the structural gene for glutamine sythetase (glnA) and in which the control of both the level and state of adenylylation of glutamine synthetase is altered. Each mutation alters a different component of the adenylylation system of glutamine synthetase [L-glutamate:ammonia ...
متن کاملChinese Hamster Liver Glutamine Synthetase
Purification procedures to prepare homogeneous glutamine synthetase from Chinese hamster liver and a sensitive radioisotope assay for the enzyme are described. The enzyme has a molecular weight of 335,000 as determined by sedimentation in a glycerol gradient, and consists of 8 identical subunits with molecular weights of 42,000 as determined by mobility in sodium dodecyl sulfate polyacrylamide ...
متن کاملOxidative Modification of Glutamine Synthetase
The first step in the proteolytic degradation of bacterial glutamine synthetase is a mixed function oxidation of one of the 16 histidine residues in the glutamine synthetase subunit (Levine, R. L. (1983) J. Biol. Chem. 258, 11823-11827). A model system, consisting of oxygen, a metal ion, and ascorbic acid, mimics the bacterial system in mediating the oxidative modification of glutamine syntheta...
متن کاملGlutamine Synthetase: Localization Dictates Outcome
Glutamine synthetase (GS) is the adenosine triphosphate (ATP)-dependent enzyme that catalyses the synthesis of glutamine by condensing ammonium to glutamate. In the circulatory system, glutamine carries ammonia from muscle and brain to the kidney and liver. In brain reduction of GS activity has been suggested as a mechanism mediating neurotoxicity in neurodegenerative disorders. In cancer, the ...
متن کاملAssay for glutamine synthetase activity.
The use of phosphoenolpyruvate plus pyruvate kinase as an ATP-generating system in the assay for glutamine synthetase activity via the formation of gamma-glutamylhydroxamate from glutamate and hydroxylamine with crude tissue preparations is shown to give values far in excess of the true glutamine synthetase activity of the tissue. This is due to the generation of pyruvate, which reacts with hyd...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
سال: 1965
ISSN: 0005-2787
DOI: 10.1016/0005-2787(65)90617-9